產(chǎn)品名稱:平板計(jì)數(shù)瓊脂
Product name:PCA
產(chǎn)品用途:用于菌落總數(shù)的測定。
Product usage: For the determination of the total number of colonies.
檢驗(yàn)原理:
Test principle:
胰蛋白胨提供碳源和氮源;酵母浸粉提供B 族維生素;葡萄糖提供能源;瓊脂是培養(yǎng)基的凝固劑。
Tryptone provides carbon and nitrogen sources. Yeast extract powder provides B vitamins; Glucose provides energy; AGAR is a coagulant for medium.
配方成分
Formula ingredients
配方 Formula |
含量(g/L) Content (g/L) |
胰蛋白胨 tryptone |
5.0 |
酵母浸粉 Yeast infusion powder |
2.5 |
葡萄糖 Glucose |
1.0 |
瓊脂 Agar |
15.0 |
pH |
7.0 ± 0.2 25℃ |
使用方法
Usage
稱取本品 23.5g,加熱攪拌溶解于1000ml 蒸餾水中,分裝試管或錐形瓶,121℃高壓滅菌15分鐘,備用。
Weigh 23.5g of this product, stir and dissolve it in 1000ml distilled water, divide into test tubes or conical bottles, autoclave at 121℃ for 15 minutes, and set aside.
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