產(chǎn)品名稱:mEC肉湯
Product name:mE.Coli Broth
產(chǎn)品用途:用于大腸桿菌O157 :H7選擇性增菌培養(yǎng)。
Product usage: Used for selective enhancement culture of Escherichia coli O157 :H7.
配方成分
Formula ingredients
配方 Formula |
含量(g/L) Content (g/L) |
胰蛋白胨 Tryptone |
20.0 |
乳糖 Lactose |
5.0 |
三號(hào)膽鹽 Bile Salt III |
1.12 |
磷酸氫二鉀 Dipotassium hydrogen phosphate |
4.0 |
磷酸二氫鉀 Potassium dihydrogen phosphate |
1.5 |
氯化鈉 Sodium chloride |
5.0 |
PH |
6.9±0.1 25℃ |
使用方法
Usage
稱取本品37.0g,加熱溶解于1000ml蒸餾水中,分裝三角瓶,每瓶225ml,121℃高壓滅菌15分鐘,冷卻后,加入過(guò)濾除菌的新生霉素1支(4.5mg),使最終濃度為20ug/ml混勻,加入樣品液后,放置42℃水浴培養(yǎng)18-24小時(shí)。
Weigh 37.0g of this product, heat and dissolve it in 1000ml distilled water, divided into triangular bottles, each bottle is 225ml, autoclaved at 121℃ for 15 minutes, after cooling, add 1 branch of neomycin (4.5mg) of filtered and sterilized bacteria, make the final concentration 20ug/ml and mix well. After adding the sample solution, Place in a 42℃ water bath for 18-24 hours.
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